Neuronal Cell Cultures

Dissociated primary neurons or human iPSC derived neurons grown on microelectrode arrays show several advantages that make them an optimal tool in drug discovery. The neurons in culture generate functional networks that exhibit electrical fingerprints comparable to their native brain tissue.

NeuroProof routinely cultivates neuronal cell cultures for at least 28 days in vitro (DIV), which enables us to study the maturation and developmental processes of neuronal network growth. With NeuroProof’s data analysis it is possible to resolve the developmental state with a precision of two days.

The spontaneous electrical activity of these cultures on a microelectrode array shows a complex activity pattern, which is quantitatively described by more than 200 parameters by NeuroProof's mathematical approach. This pattern can be influenced by pharmaceuticals and nutraceuticals in a distinct and reproducible manner. This rich information content can be exploited for the phenotypic screening in drug discovery.

NeuroProof offers screening services using primary neuronal network cultures derived from

• frontal cortex
• hippocampus
• amygdala/hippocampus co-culture
• midbrain
• midbrain/cortex co-culture
• spinal cord
• hypothalamus
• or human neuronal cultures derived from induced pluripotent stem cells.

Primary tissue cultures are dissected between embryonic day E14 and E18 according to the tissue. Please contact us for more information.