Glutamate Excitotoxicity Assay

Contact us for further information!

Overactivation of glutamate receptors is a fundamental mechanism in neurodegenerative diseases it impairs cellular calcium homeostasis, activates nitric oxide synthesis and generation of free radicals causing programmed cell death.

Excitotoxicity induced by glutamate serves as a dependable model in understanding neurodegenerative diseases such as Alzheimer's disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis (ALS), HIV-associated neurodegenerative disorders, and others. Excitotoxicity emerges as a common pathway in various neurodegenerative conditions, making a functional phenotypic assay an intriguing approach for targeting these diseases.
At our facility, we provide services with this assay using primary frontal cortex cultures or human iPSC-derived tri-cultures comprising glutamatergic, GABAergic neurons, and astrocytes.

Cell Culture: Experiments were performed with primary frontal cortex cultures of mouse after 28 days in vitro. 

 

glutamate excitotoxicity assay

Image: Multi-parametric characterization of neuronal activity after glutamate/vehicle pretreatment and memantine/vehicle treatment (followed for 3 hours). Mean values presented with standard error. Comparison conducted using Student’s unpaired t-test against "water + water" (*) and “glutamate 100 µM + water” (+).

More Services

In addition to the primary assay, we offer supplementary or standalone assays including:

  • Viability Assay: Utilizing the ToxiLight assay.
  • Apoptosis: Caspase 3,Caspase 9, Bcl-2, and Bax
  • Oxidative Stress Assay: Measuring 8-OHdg levels.

Explore the comprehensive suite of assays to delve deeper into neurodegenerative mechanisms and potential therapeutic interventions.

Save
Cookies user preferences
We use cookies to ensure you to get the best experience on our website. If you decline the use of cookies, this website may not function as expected.
Accept all
Decline all
Read more
Analytics
Tools used to analyze the data to measure the effectiveness of a website and to understand how it works.
Google Analytics
_ga
Accept
Decline