Substance Database of Microelectrode Array – MEA - Recordings
NeuroProof has developed several substance databases with the electrophysiological phenotypic profiles of several classes of compounds measured in different cell-systems from different brain regions such as frontal cortex, midbrain, spinal cord, hippocampus, and others with MEA recordings.
Our database with compound profiles measured with frontal cortex cultures (FC) from mouse is our most comprehensive database. The compounds are screened in a standardized experimental scheme. Each compound test is performed in an acute manner and with cumulative compound addition of five concentrations, which cover the dynamic range of a compound. All experiments were performed with the MAESTRO 48-well MEA plate system from Axion Biosystems, Atlanta, USA.
With these tests, the effective concentration of a compound can be determined. This concentration afterward can be used in disease models or other assays.
The NeuroProof MEA substance database
Our FC substance database contains experimental compounds and drugs from a wide range of indications.
NeuroProof has tested drugs for these indications or drug classes:
Alzheimer‘s disease, analgesics, antibiotics, depression, dermatitis, epilepsy, gastroprokinetic agent, high blood pressure, malaria, narcotic overdose, nausea, NSAID, Parkinson’s disease, psychosis/schizophrenia, rheumatoid arthritis, sleeping problems, and statins.
The NeuroProof FC database contains compounds with a wide range of different mode of actions as agonists or antagonists:
- AMPA receptor
- calcium homeostasis
- delta-opioid receptor
- dopamine receptor (D1, D2)
- GABA-A receptor
- GABA-B receptor
- gap junction
- glucocorticoid receptors
- glycine receptor
- HMG-CoA reductase
- Jak receptors (Jak1, Jak2, Jak3)
- kainate receptor
- mGlu2/3 receptor
- mu-opioid receptor
- muscarinic acetylcholine receptor
- Na+/K+ ATPase
- nicotic acetylcholine receptor
- NMDA receptor
- potassium channel
- serotonin receptor (5-HT1A, 5-HT2A, 5-HT2C, 5-HT2B, 5-HT3A, 5-HT4)
- SST2/5 receptor
- SST3 receptor
- voltage-gated sodium channels
- voltage-dependent calcium channels
NeuroProof offers the comparison of new compound profiles against its substance database. These findings allow us to find a potential applications for a compound, to assess their behavior against competitor’s compounds, or to get the first hint of mechanisms that the test compounds are targeting.
NeuroProof sends on request datasheets with concentration-response curves for compounds in its FC substance database.
Concentration-Reponse Profile Example
Here we present an example of a functional phenotypic profile with concentration-response curves of spike train parameters for valproic acid on primary frontal cortex cultures: